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Validation of the specifically expressed lncRNAs in HGSC in multiple samples of HGSC ( n = 22), normal fallopian tube tissue ( n = 10) and normal ovarian tissues ( n = 10). Box plots of relative expression levels of twelve <t>lncRNA</t> candidates in the twenty‐two HGSC tissue samples, ten normal fallopian tube tissue samples, and ten normal ovary tissue samples which were selected by real‐time <t>RT‐PCR</t> in duplicate using a PCR‐array. The relative expression levels were calculated using the mean value of normal fallopian tube tissues as one. GAPDH was used as an internal control. ** p < 0.01. The expression levels in 11 lncRNA candidates (ACTA2‐AS1, ADAMTS9‐AS2, CBR3‐AS1, HAND2‐AS1, IPW, LINC00312, LINC00887, MEG3, NBR2, TSIX and XIST) which were downregulated in HGSC were confirmed to be degreased in HGSC. On the other hand, the expression levels in one lncRNA candidate (LINC00152) which was upregulated in HGSC, was not validated to be upregulated in HGSC.
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 miRNA  expression levels in melanoma patients vs. controls.

Journal: Biomedicines

Article Title: Prognostic Significance of miRNA Subtypes in Melanoma: A Survival Analysis and Correlation with Treatment Response Across Patient Stages

doi: 10.3390/biomedicines12122809

Figure Lengend Snippet: miRNA expression levels in melanoma patients vs. controls.

Article Snippet: For the purpose of profiling miRNA expression, we employed the Human Cancer PathwayFinder miScript miRNA PCR Array from Qiagen, which targets 84 miRNAs known to be associated with various cancers, including melanoma.

Techniques: Expressing, Control

Kaplan–Meier survival analysis based on  miRNA  expression.

Journal: Biomedicines

Article Title: Prognostic Significance of miRNA Subtypes in Melanoma: A Survival Analysis and Correlation with Treatment Response Across Patient Stages

doi: 10.3390/biomedicines12122809

Figure Lengend Snippet: Kaplan–Meier survival analysis based on miRNA expression.

Article Snippet: For the purpose of profiling miRNA expression, we employed the Human Cancer PathwayFinder miScript miRNA PCR Array from Qiagen, which targets 84 miRNAs known to be associated with various cancers, including melanoma.

Techniques: Expressing

Kaplan–Meier survival analysis based on miRNA expression.

Journal: Biomedicines

Article Title: Prognostic Significance of miRNA Subtypes in Melanoma: A Survival Analysis and Correlation with Treatment Response Across Patient Stages

doi: 10.3390/biomedicines12122809

Figure Lengend Snippet: Kaplan–Meier survival analysis based on miRNA expression.

Article Snippet: For the purpose of profiling miRNA expression, we employed the Human Cancer PathwayFinder miScript miRNA PCR Array from Qiagen, which targets 84 miRNAs known to be associated with various cancers, including melanoma.

Techniques: Expressing

Association between  miRNA  expression and treatment response.

Journal: Biomedicines

Article Title: Prognostic Significance of miRNA Subtypes in Melanoma: A Survival Analysis and Correlation with Treatment Response Across Patient Stages

doi: 10.3390/biomedicines12122809

Figure Lengend Snippet: Association between miRNA expression and treatment response.

Article Snippet: For the purpose of profiling miRNA expression, we employed the Human Cancer PathwayFinder miScript miRNA PCR Array from Qiagen, which targets 84 miRNAs known to be associated with various cancers, including melanoma.

Techniques: Expressing

 miRNA  expression across patient stages.

Journal: Biomedicines

Article Title: Prognostic Significance of miRNA Subtypes in Melanoma: A Survival Analysis and Correlation with Treatment Response Across Patient Stages

doi: 10.3390/biomedicines12122809

Figure Lengend Snippet: miRNA expression across patient stages.

Article Snippet: For the purpose of profiling miRNA expression, we employed the Human Cancer PathwayFinder miScript miRNA PCR Array from Qiagen, which targets 84 miRNAs known to be associated with various cancers, including melanoma.

Techniques: Expressing

Correlation between  miRNA  expression and Breslow Thickness.

Journal: Biomedicines

Article Title: Prognostic Significance of miRNA Subtypes in Melanoma: A Survival Analysis and Correlation with Treatment Response Across Patient Stages

doi: 10.3390/biomedicines12122809

Figure Lengend Snippet: Correlation between miRNA expression and Breslow Thickness.

Article Snippet: For the purpose of profiling miRNA expression, we employed the Human Cancer PathwayFinder miScript miRNA PCR Array from Qiagen, which targets 84 miRNAs known to be associated with various cancers, including melanoma.

Techniques: Expressing

Validation of the specifically expressed lncRNAs in HGSC in multiple samples of HGSC ( n = 22), normal fallopian tube tissue ( n = 10) and normal ovarian tissues ( n = 10). Box plots of relative expression levels of twelve lncRNA candidates in the twenty‐two HGSC tissue samples, ten normal fallopian tube tissue samples, and ten normal ovary tissue samples which were selected by real‐time RT‐PCR in duplicate using a PCR‐array. The relative expression levels were calculated using the mean value of normal fallopian tube tissues as one. GAPDH was used as an internal control. ** p < 0.01. The expression levels in 11 lncRNA candidates (ACTA2‐AS1, ADAMTS9‐AS2, CBR3‐AS1, HAND2‐AS1, IPW, LINC00312, LINC00887, MEG3, NBR2, TSIX and XIST) which were downregulated in HGSC were confirmed to be degreased in HGSC. On the other hand, the expression levels in one lncRNA candidate (LINC00152) which was upregulated in HGSC, was not validated to be upregulated in HGSC.

Journal: Reproductive Medicine and Biology

Article Title: Identification of long noncoding RNAs downregulated specifically in ovarian high‐grade serous carcinoma

doi: 10.1002/rmb2.12572

Figure Lengend Snippet: Validation of the specifically expressed lncRNAs in HGSC in multiple samples of HGSC ( n = 22), normal fallopian tube tissue ( n = 10) and normal ovarian tissues ( n = 10). Box plots of relative expression levels of twelve lncRNA candidates in the twenty‐two HGSC tissue samples, ten normal fallopian tube tissue samples, and ten normal ovary tissue samples which were selected by real‐time RT‐PCR in duplicate using a PCR‐array. The relative expression levels were calculated using the mean value of normal fallopian tube tissues as one. GAPDH was used as an internal control. ** p < 0.01. The expression levels in 11 lncRNA candidates (ACTA2‐AS1, ADAMTS9‐AS2, CBR3‐AS1, HAND2‐AS1, IPW, LINC00312, LINC00887, MEG3, NBR2, TSIX and XIST) which were downregulated in HGSC were confirmed to be degreased in HGSC. On the other hand, the expression levels in one lncRNA candidate (LINC00152) which was upregulated in HGSC, was not validated to be upregulated in HGSC.

Article Snippet: The cDNA was analyzed using an RT2 lncRNA PCR Array Human Cancer PathwayFinder (Qiagen, GeneGlobe ID LAHS‐002Z), which can analyze 84 types of lncRNAs reported to be related to various cancers.

Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR, Control